cell lines human esc line wa09 wicell Search Results


cell lines h9 hesc wicell wa09 r1 mesc atcc scrc 1011 h9 c8  (ATCC)
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Cell Lines H9 Hesc Wicell Wa09 R1 Mesc Atcc Scrc 1011 H9 C8, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cell lines wa09 h9 human escs wicell wae009 a wtc11 human ipscs laboratory  (WiCell Research Institute Inc)
99
WiCell Research Institute Inc cell lines wa09 h9 human escs wicell wae009 a wtc11 human ipscs laboratory
Cell Lines Wa09 H9 Human Escs Wicell Wae009 A Wtc11 Human Ipscs Laboratory, supplied by WiCell Research Institute Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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esc cell lines  (WiCell Research Institute Inc)
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WiCell Research Institute Inc esc cell lines
Esc Cell Lines, supplied by WiCell Research Institute Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hescs lines wa01  (Coriell Institute for Medical Research)
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human embryonic stem cells hescs  (WiCell Research Institute Inc)
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WiCell Research Institute Inc human embryonic stem cells hescs
Human Embryonic Stem Cells Hescs, supplied by WiCell Research Institute Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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wa09-pcbc  (wicell research institute inc)
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wicell research institute inc wa09-pcbc
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wa09 lt2e h9caggfp p80 xx  (WiCell Research Institute Inc)
93
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Similarities between male and female hESC in neuronal differentiation: (A) Overview of the experimental procedure: Four hESC lines of each sex were differentiated for 37 days to a population of mixed neurons induced by small molecules in 2D culture. Bulk RNA sequencing and qPCR. (B) A PCA analysis of expressed genes displays similar gene expression patterns at D0, some inter-cell line differences during early differentiation, and a sex-biased separation at D37 (XY: WA14, HS401, HS983a, XX: <t>WA09,</t> HS975, HS980). (C) Marker gene expression for pluripotency (OCT4, NANOG), ectoderm (PAX6, SOX1), immature neurons (DCX, NCAM), mature neurons (MAP2, DLG4, SYP), post-mitotic neurons (ELAVL2, TUBA1A, NDN), as well as for proliferation (MKI67, PCNA), and apoptosis (CASP3, BCL2) show a similar expression in male and female cell lines. (D) Markers for specialized neurons (GABAergic, Dopaminergic, Glutamatergic) display a premature state of the neurons after 37 days of differentiation. (E) A gene set enrichment analysis at D4, D9, and D37 reveals an upregulation of biological processes implicated in neuronal differentiation in all cell lines. The GSEA was summarized in an enrichment map. Each dot represents a gene set; the size of the dot corresponds to the size of the gene set. If the dots are connected or in close proximity, the more the gene sets are overlapping, and the closer related are their associated biological functions. Gene sets were grouped by a common GO-term classification and named after an overarching biological function. Biological functions implicated in neuronal development are highlighted in blue. Gene sets are filtered by FDR q-values <0.1. created with BioRender.com .
Wa09 Lt2e H9caggfp P80 Xx, supplied by WiCell Research Institute Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human embryonic stem cells  (WiCell Research Institute Inc)
95
WiCell Research Institute Inc human embryonic stem cells
Similarities between male and female hESC in neuronal differentiation: (A) Overview of the experimental procedure: Four hESC lines of each sex were differentiated for 37 days to a population of mixed neurons induced by small molecules in 2D culture. Bulk RNA sequencing and qPCR. (B) A PCA analysis of expressed genes displays similar gene expression patterns at D0, some inter-cell line differences during early differentiation, and a sex-biased separation at D37 (XY: WA14, HS401, HS983a, XX: <t>WA09,</t> HS975, HS980). (C) Marker gene expression for pluripotency (OCT4, NANOG), ectoderm (PAX6, SOX1), immature neurons (DCX, NCAM), mature neurons (MAP2, DLG4, SYP), post-mitotic neurons (ELAVL2, TUBA1A, NDN), as well as for proliferation (MKI67, PCNA), and apoptosis (CASP3, BCL2) show a similar expression in male and female cell lines. (D) Markers for specialized neurons (GABAergic, Dopaminergic, Glutamatergic) display a premature state of the neurons after 37 days of differentiation. (E) A gene set enrichment analysis at D4, D9, and D37 reveals an upregulation of biological processes implicated in neuronal differentiation in all cell lines. The GSEA was summarized in an enrichment map. Each dot represents a gene set; the size of the dot corresponds to the size of the gene set. If the dots are connected or in close proximity, the more the gene sets are overlapping, and the closer related are their associated biological functions. Gene sets were grouped by a common GO-term classification and named after an overarching biological function. Biological functions implicated in neuronal development are highlighted in blue. Gene sets are filtered by FDR q-values <0.1. created with BioRender.com .
Human Embryonic Stem Cells, supplied by WiCell Research Institute Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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h9ingfphes cells  (WiCell Research Institute Inc)
93
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Similarities between male and female hESC in neuronal differentiation: (A) Overview of the experimental procedure: Four hESC lines of each sex were differentiated for 37 days to a population of mixed neurons induced by small molecules in 2D culture. Bulk RNA sequencing and qPCR. (B) A PCA analysis of expressed genes displays similar gene expression patterns at D0, some inter-cell line differences during early differentiation, and a sex-biased separation at D37 (XY: WA14, HS401, HS983a, XX: <t>WA09,</t> HS975, HS980). (C) Marker gene expression for pluripotency (OCT4, NANOG), ectoderm (PAX6, SOX1), immature neurons (DCX, NCAM), mature neurons (MAP2, DLG4, SYP), post-mitotic neurons (ELAVL2, TUBA1A, NDN), as well as for proliferation (MKI67, PCNA), and apoptosis (CASP3, BCL2) show a similar expression in male and female cell lines. (D) Markers for specialized neurons (GABAergic, Dopaminergic, Glutamatergic) display a premature state of the neurons after 37 days of differentiation. (E) A gene set enrichment analysis at D4, D9, and D37 reveals an upregulation of biological processes implicated in neuronal differentiation in all cell lines. The GSEA was summarized in an enrichment map. Each dot represents a gene set; the size of the dot corresponds to the size of the gene set. If the dots are connected or in close proximity, the more the gene sets are overlapping, and the closer related are their associated biological functions. Gene sets were grouped by a common GO-term classification and named after an overarching biological function. Biological functions implicated in neuronal development are highlighted in blue. Gene sets are filtered by FDR q-values <0.1. created with BioRender.com .
H9ingfphes Cells, supplied by WiCell Research Institute Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human embryonic stem cells h9 hesc wicell wa09 human  (ATCC)
95
ATCC human embryonic stem cells h9 hesc wicell wa09 human
Similarities between male and female hESC in neuronal differentiation: (A) Overview of the experimental procedure: Four hESC lines of each sex were differentiated for 37 days to a population of mixed neurons induced by small molecules in 2D culture. Bulk RNA sequencing and qPCR. (B) A PCA analysis of expressed genes displays similar gene expression patterns at D0, some inter-cell line differences during early differentiation, and a sex-biased separation at D37 (XY: WA14, HS401, HS983a, XX: <t>WA09,</t> HS975, HS980). (C) Marker gene expression for pluripotency (OCT4, NANOG), ectoderm (PAX6, SOX1), immature neurons (DCX, NCAM), mature neurons (MAP2, DLG4, SYP), post-mitotic neurons (ELAVL2, TUBA1A, NDN), as well as for proliferation (MKI67, PCNA), and apoptosis (CASP3, BCL2) show a similar expression in male and female cell lines. (D) Markers for specialized neurons (GABAergic, Dopaminergic, Glutamatergic) display a premature state of the neurons after 37 days of differentiation. (E) A gene set enrichment analysis at D4, D9, and D37 reveals an upregulation of biological processes implicated in neuronal differentiation in all cell lines. The GSEA was summarized in an enrichment map. Each dot represents a gene set; the size of the dot corresponds to the size of the gene set. If the dots are connected or in close proximity, the more the gene sets are overlapping, and the closer related are their associated biological functions. Gene sets were grouped by a common GO-term classification and named after an overarching biological function. Biological functions implicated in neuronal development are highlighted in blue. Gene sets are filtered by FDR q-values <0.1. created with BioRender.com .
Human Embryonic Stem Cells H9 Hesc Wicell Wa09 Human, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ipsc df19 9 11t h  (WiCell Research Institute Inc)
95
WiCell Research Institute Inc ipsc df19 9 11t h
Similarities between male and female hESC in neuronal differentiation: (A) Overview of the experimental procedure: Four hESC lines of each sex were differentiated for 37 days to a population of mixed neurons induced by small molecules in 2D culture. Bulk RNA sequencing and qPCR. (B) A PCA analysis of expressed genes displays similar gene expression patterns at D0, some inter-cell line differences during early differentiation, and a sex-biased separation at D37 (XY: WA14, HS401, HS983a, XX: <t>WA09,</t> HS975, HS980). (C) Marker gene expression for pluripotency (OCT4, NANOG), ectoderm (PAX6, SOX1), immature neurons (DCX, NCAM), mature neurons (MAP2, DLG4, SYP), post-mitotic neurons (ELAVL2, TUBA1A, NDN), as well as for proliferation (MKI67, PCNA), and apoptosis (CASP3, BCL2) show a similar expression in male and female cell lines. (D) Markers for specialized neurons (GABAergic, Dopaminergic, Glutamatergic) display a premature state of the neurons after 37 days of differentiation. (E) A gene set enrichment analysis at D4, D9, and D37 reveals an upregulation of biological processes implicated in neuronal differentiation in all cell lines. The GSEA was summarized in an enrichment map. Each dot represents a gene set; the size of the dot corresponds to the size of the gene set. If the dots are connected or in close proximity, the more the gene sets are overlapping, and the closer related are their associated biological functions. Gene sets were grouped by a common GO-term classification and named after an overarching biological function. Biological functions implicated in neuronal development are highlighted in blue. Gene sets are filtered by FDR q-values <0.1. created with BioRender.com .
Ipsc Df19 9 11t H, supplied by WiCell Research Institute Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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h9 wa09 wicell  (WiCell Research Institute Inc)
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WiCell Research Institute Inc h9 wa09 wicell
Similarities between male and female hESC in neuronal differentiation: (A) Overview of the experimental procedure: Four hESC lines of each sex were differentiated for 37 days to a population of mixed neurons induced by small molecules in 2D culture. Bulk RNA sequencing and qPCR. (B) A PCA analysis of expressed genes displays similar gene expression patterns at D0, some inter-cell line differences during early differentiation, and a sex-biased separation at D37 (XY: WA14, HS401, HS983a, XX: <t>WA09,</t> HS975, HS980). (C) Marker gene expression for pluripotency (OCT4, NANOG), ectoderm (PAX6, SOX1), immature neurons (DCX, NCAM), mature neurons (MAP2, DLG4, SYP), post-mitotic neurons (ELAVL2, TUBA1A, NDN), as well as for proliferation (MKI67, PCNA), and apoptosis (CASP3, BCL2) show a similar expression in male and female cell lines. (D) Markers for specialized neurons (GABAergic, Dopaminergic, Glutamatergic) display a premature state of the neurons after 37 days of differentiation. (E) A gene set enrichment analysis at D4, D9, and D37 reveals an upregulation of biological processes implicated in neuronal differentiation in all cell lines. The GSEA was summarized in an enrichment map. Each dot represents a gene set; the size of the dot corresponds to the size of the gene set. If the dots are connected or in close proximity, the more the gene sets are overlapping, and the closer related are their associated biological functions. Gene sets were grouped by a common GO-term classification and named after an overarching biological function. Biological functions implicated in neuronal development are highlighted in blue. Gene sets are filtered by FDR q-values <0.1. created with BioRender.com .
H9 Wa09 Wicell, supplied by WiCell Research Institute Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Similarities between male and female hESC in neuronal differentiation: (A) Overview of the experimental procedure: Four hESC lines of each sex were differentiated for 37 days to a population of mixed neurons induced by small molecules in 2D culture. Bulk RNA sequencing and qPCR. (B) A PCA analysis of expressed genes displays similar gene expression patterns at D0, some inter-cell line differences during early differentiation, and a sex-biased separation at D37 (XY: WA14, HS401, HS983a, XX: WA09, HS975, HS980). (C) Marker gene expression for pluripotency (OCT4, NANOG), ectoderm (PAX6, SOX1), immature neurons (DCX, NCAM), mature neurons (MAP2, DLG4, SYP), post-mitotic neurons (ELAVL2, TUBA1A, NDN), as well as for proliferation (MKI67, PCNA), and apoptosis (CASP3, BCL2) show a similar expression in male and female cell lines. (D) Markers for specialized neurons (GABAergic, Dopaminergic, Glutamatergic) display a premature state of the neurons after 37 days of differentiation. (E) A gene set enrichment analysis at D4, D9, and D37 reveals an upregulation of biological processes implicated in neuronal differentiation in all cell lines. The GSEA was summarized in an enrichment map. Each dot represents a gene set; the size of the dot corresponds to the size of the gene set. If the dots are connected or in close proximity, the more the gene sets are overlapping, and the closer related are their associated biological functions. Gene sets were grouped by a common GO-term classification and named after an overarching biological function. Biological functions implicated in neuronal development are highlighted in blue. Gene sets are filtered by FDR q-values <0.1. created with BioRender.com .

Journal: Frontiers in Cell and Developmental Biology

Article Title: Sex-biased gene expression during neural differentiation of human embryonic stem cells

doi: 10.3389/fcell.2024.1341373

Figure Lengend Snippet: Similarities between male and female hESC in neuronal differentiation: (A) Overview of the experimental procedure: Four hESC lines of each sex were differentiated for 37 days to a population of mixed neurons induced by small molecules in 2D culture. Bulk RNA sequencing and qPCR. (B) A PCA analysis of expressed genes displays similar gene expression patterns at D0, some inter-cell line differences during early differentiation, and a sex-biased separation at D37 (XY: WA14, HS401, HS983a, XX: WA09, HS975, HS980). (C) Marker gene expression for pluripotency (OCT4, NANOG), ectoderm (PAX6, SOX1), immature neurons (DCX, NCAM), mature neurons (MAP2, DLG4, SYP), post-mitotic neurons (ELAVL2, TUBA1A, NDN), as well as for proliferation (MKI67, PCNA), and apoptosis (CASP3, BCL2) show a similar expression in male and female cell lines. (D) Markers for specialized neurons (GABAergic, Dopaminergic, Glutamatergic) display a premature state of the neurons after 37 days of differentiation. (E) A gene set enrichment analysis at D4, D9, and D37 reveals an upregulation of biological processes implicated in neuronal differentiation in all cell lines. The GSEA was summarized in an enrichment map. Each dot represents a gene set; the size of the dot corresponds to the size of the gene set. If the dots are connected or in close proximity, the more the gene sets are overlapping, and the closer related are their associated biological functions. Gene sets were grouped by a common GO-term classification and named after an overarching biological function. Biological functions implicated in neuronal development are highlighted in blue. Gene sets are filtered by FDR q-values <0.1. created with BioRender.com .

Article Snippet: The human embryonic stem cell lines WA14 p33 (XY) ( ) and WA09 LT2e-H9CAGGFP p80 (XX) ( ) were acquired from WiCell, Madison, Wisconsin.

Techniques: RNA Sequencing Assay, Expressing, Marker

hESC lines and gene expression analysis method and timepoints.

Journal: Frontiers in Cell and Developmental Biology

Article Title: Sex-biased gene expression during neural differentiation of human embryonic stem cells

doi: 10.3389/fcell.2024.1341373

Figure Lengend Snippet: hESC lines and gene expression analysis method and timepoints.

Article Snippet: The human embryonic stem cell lines WA14 p33 (XY) ( ) and WA09 LT2e-H9CAGGFP p80 (XX) ( ) were acquired from WiCell, Madison, Wisconsin.

Techniques: Expressing